Deputy Head FACS Lab LRI-Cancer Research UK 1994 - 1999 5 años. The facility includes a cut-up area, processor/embedder, cryostat and microtomes. Flow Cytometry Core 110 Gene Expression Core 111 Genomics Core 111 Hybridoma Core 112 Imaging Core 112 Mass Spectometry II Core 113 ... (LRI) experienced banner years in 2004 and 2005 with respect to both external peer-reviewed funding and publications in the highest impact journals. Engraftment of BM… Flow cytometry. Amernoon(parallel(session(from(14:00(–16:00(atIMS(level(7(CR Newtechnologiesbyindustrialapplicaonspecialists (10min(presentaons)! We acknowledge Cathy Shemo and Anne Cotleur for help with flow cytometry and phlebotomy, Jerome Wujek and Bruce Trapp for iba-1 monoclonal antibody and helpful comments about the data, Judith Drazba and the LRI Confocal Core for excellent technical assistance, and members of the Ransohoff lab for advice and suggestions. 1 Coronavirus: Find the latest articles and preprints FlowJo License Requests (Group License shared through LRI Flow Cytometry Core Service) Lab Requesting License (PI Name) Requestor Computer to be registered (LRICS number) Certification by Requestor I understand that: • The FlowJo license will be associated with the above computer, not with any particular user. Immunohistochemistry. Our faculty direct state-of-the-art cores such as the Flow Cytometry and Sorting. Core Courses Expand all Medical Microbiology (PAMB D650) We thank Angela Leschinsky for her assistance in clinical sample collection, the LRI Flow Cytometry Core for assistance with sorting experiments, and Dr. Adrian Hernandez for his assistance with sample size calculations. Cells were sorted using a 100 µm nozzle size at a low sheath pressure of 20 psi. 8, 17 Briefly, cultured MSCs were harvested, washed, and resuspended in flow cytometry cell sorting buffer (1% bovine serum albumin and 0.1% sodium azide in 1 × Hank's balanced salt solution). Flow Cytometry Dipti Vachi . The Lupus Research Institute (LRI) invites applications for its Dr. William E. Paul Distinguished Innovator Awards in Lupus and Immunology, a global program that will provide outstanding scientists with substantial support for up to four years to conduct novel research into the fundamental causes of lupus and so provide new directions toward a cure or prevention. Access. Amernoon!parallel!session!from!14:00!–16:00!atIMS!level!7!CR Newtechnologiesbyindustrialapplicaonspecialists! Flow sorting was done using a BD FACS Aria II sorter and BD FACS Diva software (BD Biosciences) with technical assistance from personnel at the Cleveland Clinic LRI Flow Cytometry Core.

Chairman: Jaroslaw Maciejewski, MD, PhD, FACP

The Department of Translational Hematology and Oncology Research (THOR) conducts cancer research to develop and make available novel diagnostic tools, targeted therapies and clinical trials for direct use in patient care. Derek Davies is the Flow Cytometry Science Technology Platform (STP) Training Lead at the Francis Crick Institute in London, UK and has been a leader in flow cytometry for over 30 years. Abstract Beta‐adrenergic receptors (β‐ARs) play a critical role in many diseases. quantitative studies of cells and extracellular vesicles, and single-cell gene transcriptome analyses . Cleveland Clinic LRI Flow Cytometry Core 9500 Euclid Avenue Cleveland, Ohio 44195 The Flow Cytometry Core provides investigators with a resource for analytical and preparative studies of cells using flow cytometry. This work was supported by funding from National Cancer Institute at the NIH (CA126847 to T.V.B.) Legal Eight to 10 weeks after last injection, mice were killed for flow cytometry or brain histology. W. Zhu, and we particularly appreciate the aid of Judy Drazba of . Paraffin wax blocks and frozen tissue sectioning. Macrophages or ... We also appreciate the helpful assistance of S. O'Bryant of the LRI Flow Cytometry Core and particularly appreciate the aid of J. Drazba of the Imaging Core for TIRF microscopy. The collective efforts of my Core resulted in a grand mean score above the LRI average. Cutting is generally undertaken on standard blocks but megablocks can also be cut. Naive CD4 + T cells were isolated by flow cytometry based on the markers CD4 + CD62L + CD44 − CD25 − or using the Easysep mouse naive T cell isolation kit. Flow cytometry was performed as previously described 3 with modification. Visit to the Flow Core Lab LRI June 19th 11:30-12pm Cathy Shemo, MS/ Reda Mahfouz, MD Batch 2 Visit to the Flow Core Lab LRI June 20th 10.00am Amr Kader, MD Normal Fertilization, Manager of the FACS Laboratory, London Research Institute (LRI), Cancer Research UK.. Derek’s first post after graduating was in a regional Cytopathology Unit where, amongst other duties, he was introduced to cytometry - using a microdensitometer to measure DNA content in cervical cells. Optimized protocol for the preparation of single cells from cutaneous wounds for flow cytometric cell sorting and analysis of macrophages. We also thank J. Drazba along with M. Yin for transmission electron microscopy. Our other shared resources comprise cutting-edge equipment and technology for Advanced Microscopy, –Omics (Genomics, Epigenomics, Transcriptomics and Microbiome technology) and Metabolic Profiling studies. IV THE ROLE OF BCL-2 FAMILY IN CLINICAL RESPONSE OF CHRONIC LYMPHOCYTIC LEUKEMIA SAYER ALHARBI ABSTRACT The anti-apoptotic Bcl-2 proteins regulate lymphocyte survival and are over- Derek Davies, London Research Institute,UK. of the LRI Flow Cytometry Core, the transfer of several mice from . Flow cytometry. Flow Cytometry Consultation Flow Cytometry Kewal Asosingh, Ph.D., Staff, Core Director Location: NB2-28a Phone: 216-444-0891 Email: asosink@ccf.org Assistance with panel design, controls, troubleshooting, data analysis and interpretation, grant writing, budget, generation of publication quality figure according to the MSCs were expanded to passage 4 and were examined for expression of specific surface markers using flow cytometry as previously described.

Based on the most current scientific standards, THOR researchers apply advances in the laboratory to … high throughput and capillary sequencing, genotyping, gene expression, methylation, structural variation . Leicester Royal Infirmary Leicester LE2 7LX. H & E staining. Briefly, peripheral erythrocytes were lysed and washed in FACS buffer. Roy!Bongaerts,SonyBiotechnologyEurope range of special stains. Providing a comprehensive range of histological techniques. Successfully maintain Flow Cytometry Core budget through careful planning and management of expenses. Head of Flow Cytometry Unit ... Flow Cytometrist UCL 2000 - 2006 6 años. Genomics . the Imaging Core and our many blood donors. Flow cytometry using procaspase activating compound-1 (PAC-1) antibody binding to platelets treated or not with b-AP15 and stimulated or not with 0.2 U of thrombin (n=3). The existing flow cytometry / FACS labs at the former Cancer Research UK, London Research Institute (LRI) and the MRC's National Institute for Medical Research (NIMR) will be joining forces to become the biggest (and we hope best) flow cytometry core facility in Europe. BD BioScience(TBC) Beckman!Coulter(TBC)! Accumulating evidence shows a causative role for the bone marrow (BM) in the genesis and progression of pulmonary hypertension (PH). The LRI is Internal and external ; Expertise Core Facility Tissue collection, fixation, processing. Using iLab Operations Software Core Admin/Staff Core Customers Lab Administrators Requisitioning. Conjugate formation was monitored by flow cytometry for B220 + Thy1 + cells, gating on the DP population. ... and the Lab continued to grow. Flow Cytometry . The existing flow cytometry / FACS labs at the former Cancer Research UK, London Research Institute (LRI) and the MRC's National Institute for Medical Research (NIMR) will be joining forces to become the biggest (and we hope best) flow cytometry core facility in Europe. Glass Washing and Autoclaving . N. Gupta is a PhD

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